Colorimetric Microdetermination of Free Fatty Acids1
نویسندگان
چکیده
A colorimetric method for determination of free fatty acids has been developed, based on the estimation of copper in a chloroform extract of their cupric salts with oxalic acid bis-(cyclohexylidenehydrazide). Contamination with inorganic copper during extraction is practically eliminated by using filter-paper pads. Specificity and interferences are discussed. Recoveries average 101.1%. Normal values are 10.0 ± 8.2 mg/dl (0.390 ± 0.320 mmol/liter), with no statistically significant sex-related difference. Correlation with the reference method of Dole and Meinertz [J. Biol. Chem. 235, 2595 (1960)] is 0.954. Ten samples can be measured in about 45 mm.
منابع مشابه
AutomatedDiscreteKineticMethodfor ErythrocyteAcetyicholinesterase and PlasmaCholinesterase
926 CLINICAL CHEMISTRY, Vol. 27, No. 6, 1981 1. Broderson, R., Prevention of kernicterus, based on recent progress in bilirubin chemistry. Acta Paediatr. Scand. 66, 625-634 (1977). 2. Andrew, G., Chan, G., and Schiff, D., Lipid metabolism in the neonate. I. The effects of Intralipid infusion on plasma triglyceride and free fatty acid concentrations in the neonate. J. Pediatr. 88, 273-278 (1976)...
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